Pitfalls and best practices for CRISPR/Cas9-based gene editing webinar, May 25, 2021
Bioneer is looking forward to hosting a LIVE webinar with Samplix on May 25th, on pitfalls and best practices for CRISPR/Cas9-based gene editing.
The webinar has 2 presentations on the agenda, by Dr. Benjamin Schmid from Bioneer and Dr. Peter Mouritzen from Samplix.
Presentation 1: 10 Things You Should Think Of When Designing A Good Gene Editing Strategy
Presented by Dr. Benjamin Schmid, Research Scientist, Cellular Engineering and Disease Models, Bioneer A/S
Synopsis
Bioneer A/S has been working with different genome editing techniques for almost 10 years and has been through the evolution of gene editing. During the last 5 years, CRISPR/Cas9 has been the gene editing tool of choice. Dr. Benjamin Schmid will present lessons learnt and key quality control steps during a gene editing project.
Key Learning Points
- Learn how to plan a good gene-editing strategy
- Don’t rush into it – learn about the most common pitfalls using CRISPR/Cas9
- Improved technologies and workflow to maximize success rate
- What needs to be checked after a successful gene edit?
Presentation 2: How To Ensure Your Gene Editing Validation Accounts For Larger Unintended Rearrangements
Presented by Dr. Peter Mouritzen, VP Application and Market Development, Samplix ApS
Enabling precise genetic modifications, CRISPR-based editing techniques have redefined how we explore the genome and may allow engineering tomorrow’s medicines or better crops. Validating CRISPR edits, however, is not that straightforward. Commonly used methods have missed critical unintended alterations. Dr. Peter Mouritzen discusses an expanded quality control for unintended modifications by covering a larger region around the gene edit and demonstrates this with examples to establish a comprehensive validation strategy.
- See examples of unintended alterations from CRISPR editing that can go undetected
- Discover the importance of resolving edit patterns and rearrangements
- Learn how to enrich and sequence long DNA fragments to validate CRISPR edits
The webinar is free of charge and you can register here.
