Recombinant Proteins - Mammalian

Transient Gene Expression

Transient Gene Expression (TGE) is the optimal choice for achieving rapid mammalian production, serving as the initial stage in assessing the expression of proteins in mammalian cells.

Within a timeframe as short as 5 weeks, we can provide protein for research purposes and concurrently evaluate up to 72 constructs to ascertain the feasibility of protein expression in HEK293 or CHO cells. Upon the completion of initial feasibility studies, we can scale expression up to 30 L culture volume using shake-flasks.

  • Systems: HEK293-6E, CHO-3E7, or Your Preferred System
  • Scale from 1mL to 30L
  • Test up to 72 constructs in parallel
  • As little as 5 weeks to purified protein
  • Yields up to 500 mg/L

Workflow at Bioneer

Leveraging our expertise across diverse protein types, we provide early insights into the expression of your protein and mitigate risk through a flexible step-wise project approach with Go/No-Go decision points.

Process Step

Our approach

Time
Gene Design and Plasmid Preparation
  • Gene sequence is optimized for use in selected system
  • Gene synthesis via 3rd party provider
  • Cloning into expression vector
  • Large scale, high purity plasmid preparation for transfection (Can be conducted as stand-alone service)
2 – 4 weeks
Feasibility Studies & Construct Screening
  • Screen up to 72 constructs in parallel using deep well plates
  • Test expression in shake-flasks (1 mL – 30 L)
  • Analysis via SDS-PAGE, A280 and igG quantification assay
2 – 3 weeks

Report Outlining Expression Results (Go/No Go)

Upscaled Expression
  • Upscaled expression of selected constructs
  • Up to 30 L culture volume can be run in parallel using shake-flasks
2 – 3 weeks

Reporting & Delivery of Culture Supernatant/Cell Pellets (Go/No-Go)
Purification and Downstream Process Development
  • Affinity purification for rapid delivery of purified product
  • Development of a downstream process customized for your protein
2+ Weeks
Characterization and Analysis
  • Customized analytical packages leveraging a full range of protein characterization methods
2+ Weeks
Formulation
  • Stability studies, buffer optimization, and more to create the optimal formulation for your protein
2+ Weeks

Reporting and Delivery of Purified Protein

Additional Services:

Workflow at Bioneer

Leveraging our expertise across diverse protein types, we provide early insights into the expression of your protein and mitigate risk through a flexible step-wise project approach with Go/No-Go decision points.

Our Approach:

  • Gene sequence is optimized for use in selected system
  • Gene synthesis via 3rd party provider
  • Cloning into expression vector
  • Large scale, high purity plasmid preparation for transfection (Can be conducted as stand-alone service)

Time: 2-4 Weeks

Our Approach:

  • Screen up to 72 constructs in parallel using deep well plates
  • Test expression in shake-flasks (1 mL – 30 L)
  • Analysis via SDS-PAGE, A280 and igG quantification assay

Time: 2-3 Weeks

Report Outlining Expression Results (Go/No Go)

Our Approach:

  • Upscaled expression of selected constructs
  • Up to 30 L culture volume can be run in parallel using shake-flasks

Time: 2-3 Weeks

Reporting & Delivery of Culture Supernatant/Cell Pellets/Inclusion Bodies (Go/No-Go)

Our Approach:

  • Affinity purification for rapid delivery of purified product
  • Development of a downstream process customized for your protein

Time: 2+ Weeks

Our Approach:

  • Customized analytical packages leveraging a full range of protein characterization methods

Time: 2+ Weeks

Our Approach:

  • Stability studies, buffer optimization, and more to create the optimal formulation for your protein

Time: 2+ Weeks

Reporting and Delivery of Purified Protein

Additional Services:

Plasmid Preparation

Peptide and Protein Formulation 

GMP Manufacturing

Contact us for a free consultation

Interested in learning how we can support your project or pipeline?

We´re always happy to chat.

ryan polito expression services and stran development

For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or  +4551443005 

ryan polito expression services and stran development

For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or  +4551443005