Protein Expression services

Recombinant Proteins - Microbial

Protein Expression Services and Strain Development

Get early, actionable insight into expression performance and scalability

We help you move from construct to purified protein quickly and with confidence. By combining deep expertise in microbial systems, particularly E. coli and L. Lactis, we identify the optimal strain and expression strategy tailored specifically to your protein and downstream goals.

From the very beginning, we evaluate expression levels, solubility, and cellular localization to give you critical insight into manufacturability and scalability.

This allows you to make informed decisions early, reduce development risk, and avoid costly surprises later.

Our fully documented, tech-transfer ready processes are designed for seamless transfer to your preferred CMO, giving you a strong and scalable foundation for clinical and commercial manufacturing.

Building on this foundation, our processes are fully documented and tech-transfer ready, providing a robust basis for transfer to your preferred CMO for API manufacturing when required.

In parallel, we offer in-house GMP-grade manufacturing of non-API proteins, including proteins used in cell and gene therapy manufacturing, diagnostics, and biosolutions. Our processes are designed to support seamless progression to pilot scale while maintaining scalability and regulatory readiness.

Lasse Pedersen, protein expression services

Expression Capabilities

Expression systems: E. coli, L. lactis, or your preferred system
Parallel screening of constructs and expression conditions
Purified protein in as little as 7 weeks
Gram-scale production
Development of Research Cell Banks

What you can expect

Early de-risking of your development program
Transparent timelines and clear communication
Scalable, tech-transfer ready processes
A partner experienced in taking proteins from research to manufacturing

Expression Capabilities

Expression systems: E. coli, L. lactis, or your preferred system
Parallel screening of constructs and expression conditions
Purified protein in as little as 7 weeks
Gram-scale production
Development of Research Cell Banks

What you can expect

Early de-risking of your development program
Transparent timelines and clear communication
Scalable, tech-transfer ready processes
A partner experienced in taking proteins from research to manufacturing

Workflow at Bioneer

Leveraging our expertise across diverse protein types and our comprehensive protein expression services, we provide early insights into the expression of your protein. We mitigate risk through a flexible step-wise project approach with Go/No-Go decision points.

Below you get an overview of our typical workflow.

Process Step	Our approach	Time
Gene Design and Plasmid Preparation	Gene sequence is optimized for use in selected system Gene synthesis via 3rd party provider Cloning into expression vector and transformation of expression strain.	2 – 4 weeks
Strain Selection and Banking	Screen up to 72 constructs in parallel using deep well plates Test of various strains, plasmids, and genetic constructs RCB banking	2+ weeks
Feasibility Studies	Test expression in shake-flasks (30 mL – 100 mL) Test of various expression conditions Analysis via SDS-PAGE, western blotting	2 – 3 weeks
	Report Outlining Expression Results (Go/No Go)
Upscaled Expression in Shake-Flasks	Upscaled expression of selected constructs Up to 5 L culture volume can be expressed in parallel using shake-flasks	2 – 3 weeks
Batch and Fed-batch Production & Upstream Process Development	Standardized batch and fed-batch production processes Development of Customized processes 6x 1 L glass fermentors 1x 15 L stainless steel fermentor 4x 50 L dedicated steel fermentors for manufacturing	2+ weeks
	Reporting & Delivery of Culture Supernatant/Cell Pellets/Inclusion Bodies (Go/No-Go)
Purification and Downstream Process Development	Affinity purification for rapid delivery of purified product Development of a downstream process customized for your protein Inclusion Body isolation, solubilization and refolding	2+ Weeks
Characterization and Analysis	Customized analytical packages leveraging a full range of protein characterization methods	2+ Weeks
Formulation	Stability studies, buffer optimization, and more to create the optimal formulation for your protein	2+ Weeks
	Reporting and Delivery of Purified Protein