Purification and Downstream Process Development
We specialize in developing customized downstream processes, drawing upon our profound understanding of the inherent complexities in protein purification and the unique characteristics of each protein.
We can rapidly provide materials using standardized methods such as affinity chromatography or devise intricate multistep processes that ensure purity levels surpassing 99%. With experience in developing processes for cGMP manufacturing, our processes are carefully designed for seamless transfer to manufacturing facilities. Whether you require rapid material, full-scale process development, or optimization of existing procedures, our team of dedicated experts is fully equipped to assist you every step of the way.
- Tagged and Non-tagged Methods
- Upscaling and Tangential Flow Filtration
- Process Development & Troubleshooting
- mg to g scale
- Inclusion Body Solubilization & Refolding
Variety of chromatographic techniques available
- Affinity chromatography (Protein A, L, IMAC, etc.)
- Ion exchange chromatography
- Size exclusion chromatography
- Mixed mode chromatography
- Hydrophobic interaction chromatography
- Endotoxin removal chromatography
- More!
Workflow at Bioneer
Leveraging our expertise across diverse protein types, we provide early insights into the expression of your protein and mitigate risk through a flexible step-wise project approach with Go/No-Go decision points.
Process Step |
Example techniques |
|
|---|---|---|
| Preparation, Extraction, Clarification |
|
|
|
Capture (Isolate, concentrate, and stabilize) |
|
|
| Intermediate Purification
(Remove Bulk Impurities) |
|
|
| Polishing
(Achieve final high level purity) |
|
|
| Finalizing/ Formulation
(final preparation) |
|
Our Process and Example techniques
Learn more about how we can help you with: Purification of Recombinant Proteins.
Contact us for a free consultation
Interested in learning how we can support your project or pipeline?
We´re always happy to chat.
For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or +4551443005
For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or +4551443005
