Characterization of Recombinant Proteins
We offer customizable characterization packages for the analysis of recombinant proteins, using a broad range of analytical techniques to thoroughly assess your protein’s properties.
In close collaboration with you, we design a tailored plan to evaluate key attributes such as purity, identity, structure, aggregation, and stability – whether your protein is produced in microbial or mammalian expression systems.
Our cross-disciplinary expertise further enables us to provide deeper insight into your protein’s functionality by integrating in vitro modelling, molecular histology, or formulation services where relevant.
Whether you are working in early-stage research, assay development, or biopharmaceutical development, our comprehensive characterization approach provides the data needed to support informed decision-making and move your project forward with confidence.
Why Bioneer?
With over 30 years of protein expertise, we offer a rapid project start-up and flexible timelines that adapt to your project needs.
Our robust and well-documented processes ensure reliable results, while our customized solutions make even the most complex projects manageable.
Based in the Medicon Valley, we combine the agility of a CRO/CDMO with the high quality standards you expect from a Nordic partner.
Analytical capabilities
Below we´ve listed our analytical capabilities.
Potency and Concentration
- Elisa
- Enzyme Assays
- Bradford/BCA quantification
- UV Spectrophotometry
Identity and Purity
- SDS-PAGE (native/reduced)
- Western blot
- Capillary electrophoresis (CGE, ciEF)
- UHPLC (SEC, IEX, RP)
Safety and Contaminant Testing
- Endotoxin detection (rFC fluorescence)
- Host cell DNA (qPCR)
- Host cell protein (CHO & E. Coli)
Advanced Characterization
- Mass spectrometry (Orbitrap Exploris 240)
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- Intact Mass
- Peptide Profiling
- Glycoprotein Analysis
- Mass Photometry (Refeyn TwoMP)
- Circular dichroism
- Dynamic light scattering
- Fluorescence and infrared spectroscopy
Functional Assays
- Immune models – functional readouts of immune activation, suppression, and signaling
- Cancer models – tumor cell and co-culture systems to assess efficacy and mechanism of action
- CNS models – neuronal cell-based assays to evaluate target engagement and functional response
→ Click to read more about our: in vitro models and assays. ←
Purity & Impurity Profiling
- UHPLC (SEC, IEX, HIC, RP)
- Host cell protein analysis (CHO and E. coli, Cygnus kits)
- Host cell DNA (qPCR)
- Endotoxin measurement
- Quantitative amino acid analysis (outsourced)
Quantification & Functional Testing
- Bradford and BCA assays
- UV spectrophotometry
Analytical capabilities
Below we´ve listed our analytical capabilities.
Potency and Concentration
- Elisa
- Enzyme Assays
- Bradford/BCA quantification
- UV Spectrophotometry
Identity and Purity
- SDS-PAGE (native/reduced)
- Western blot
- Capillary electrophoresis (CGE, ciEF)
- UHPLC (SEC, IEX, RP)
Safety and Contaminant Testing
- Endotoxin detection (rFC fluorescence)
- Host cell DNA (qPCR)
- Host cell protein (CHO & E. Coli)
Advanced Characterization
- Mass spectrometry (Orbitrap Exploris 240)
- Intact Mass
- Peptide Profiling
- Glycoprotein Analysis
- Mass Photometry (Refeyn TwoMP)
- Circular dichroism
- Dynamic light scattering
- Fluorescence and infrared spectroscopy
Functional Assays
- Immune models – functional readouts of immune activation, suppression, and signaling
- Cancer models – tumor cell and co-culture systems to assess efficacy and mechanism of action
- CNS models – neuronal cell-based assays to evaluate target engagement and functional response
→ Click to read more about our: in vitro models and assays. ←
Purity & Impurity Profiling
- UHPLC (SEC, IEX, HIC, RP)
- Host cell protein analysis (CHO and E. coli, Cygnus kits)
- Host cell DNA (qPCR)
- Endotoxin measurement
- Quantitative amino acid analysis (outsourced)
Quantification & Functional Testing
- Bradford and BCA assays
- UV spectrophotometry
How we work
We work in close dialogue with you to ensure that the analytical strategy aligns with your development and scientific goals.
Our approach is transparent and pragmatic, we provide direct, honest feedback on results and their implications, enabling you to make informed decisions at every stage.
Flexibility is central to our way of working. Analytical plans can be adjusted as new data emerges, ensuring that resources are focused where they create the most value.
Throughout the project, you will have a project manager and direct access to the scientists involved, ensuring efficient communication and a clear understanding of both progress and outcomes.
Related Services
Click to read about our:→ Microbial expression systems ←
Click to read about our: → Mammalian expression systems ←
Complementary Services to Characterization of Recombinant Proteins
When deeper biological validation is required, we offer:
These services enable functional validation beyond analytical characterization.
Our Recombinant Protein Leadership:
- Søren Madsen, Protein Expression, Dept. Head, Ph.D
Our Recombinant Protein Leadership:
- Søren Madsen, Protein Expression, Dept. Head, Ph.D
Contact us for a free consultation
Interested in understanding your protein’s structure, stability, or functionality?
We´re always happy to chat.
For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or +4551443005
For further information please contact Sr. Business Development Manager Ryan Polito on rpo@bioneer.dk or +4551443005
