R&D Services
At Bioneer, we understand the distinctiveness of each protein and advocate for tailored approaches to protein manufacturing. By leveraging our experience in protein expression, purification and characterization, gained from over 350 recombinant protein projects spanning from antibodies to industrial enzymes, we help take your recombinant protein from idea to manufactured product. With a versatile service portfolio, we guide you through the entirety of development, enhance current processes, and offer solutions to encountered hurdles. Our expertise extends to both mammalian and microbial expression systems, enabling us to determine the most suitable expression system for your protein.
Types of Proteins Previously Expressed
- mAbs
- Fabs
- Bispecifics
- Vaccine Antigens
- Enzymes
- Cytokines
- Allergens
- Fusion Proteins
- Membrane Proteins
- Hormones
- Growth Factors
Microbial Expression Systems
With over 30 years of experience working with microbial expression systems, we are poised to support you with even the most challenging projects. We develop robust and well-documented processes primed for manufacturing in Bioneer CDMO facilities or for tech transfer to your third-party CMO partner of choice.
From Gene to Manufacturing
Whether you are starting with a gene, strain, or partially developed process we can help. With flexible entry and exit points we can guide you through a portion, or the entirety, of your development journey, enhance current processes, and offer solutions to encountered hurdles.
Our extensive experience encompasses various bacterial and yeast expression systems, with specialized knowledge in E. coli and L. lactis systems. We provide access to commercially available expression systems as well as to our proprietary platforms, the Bioneer E-c System™ for E. coli and the Bioneer Lactococcus lactis P170 System™. We can also work with your preferred microbial expression system.
- Gram Yields
- As Little as 5 weeks to purified protein
- E. coli, L. lactis, and More
- Batch and Fed-batch Fermentation
- 1 L, 15 L, and 50 L reactors
Is a microbial system right for me?
Microbial protein expression offers rapid, cost-effective production of recombinant proteins at large scales due to the fast growth rate of microbial hosts compared to mammalian hosts. Microbial hosts excel in applications requiring quick protein production, such as vaccine development, simple therapeutic proteins, enzymes, or industrial bioproducts. However, microbial protein expression may not be suitable for proteins requiring complex post-translational modifications or for applications where mammalian-like glycosylation patterns are crucial. Additionally, expression often leads to insolubility or improper folding which can complicate downstream processing.
If you are in doubt, our experts can help you select the correct system. For further information, please contact Business Development Manager, Ryan Jeffrey Polito by phone or email.
Additional Services:
Mammalian Expression Systems
With over 15 years of experience working with mammalian expression systems, we are poised to support you with even the most challenging projects. We develop robust and well-documented processes for seamless transfer to a CMO facility of your choice. With a flexible service offering and cross-disciplinary approach, we act as your research partner capable of not just providing proteins, processes, and characterized cell lines, but also of tackling novel challenges and projects.
From Gene to Manufacturing
Whether you are starting with a gene, cell line, or partially developed process, we can help. With flexible entry and exit points, we can guide you through a portion, or the entirety, of your development journey, enhance current processes, and offer solutions to encountered hurdles.
We provide access to commercially available HEK293 and CHO-DG44/S systems and to our proprietary Bioneer BION-CHO 6.0 System™.
We can also work with your preferred expression system.
- Yields up to 6 g/L
- As little as 5 weeks to purified protein
- CHO-S, CHO-DG44, BION-CHO 6.0 System™, and HEK293
- Transient Gene Expression
- Cell Line Development
- Single Use Bioreactors
Is a mammalian system right for me?
Mammalian expression systems are typically chosen when protein folding, post-translational modifications, or protein-protein interactions are critical for the function of the protein of interest. They are ideal for producing complex proteins for therapeutic use such as antibodies, enzymes, or signaling molecules that require post-translational modifications like glycosylation. Additionally, mammalian systems facilitate the expression of soluble proteins and do not produce endotoxins which can simplify downstream processes. However, mammalian expression systems may not be the best choice when cost-effectiveness and speed is a primary concern, such as for enzymes for industrial applications.
If you are in doubt, our experts can help you select the correct system. For further information, please contact Business Development Manager, Ryan Jeffrey Polito by phone or email.
