Molecular Histology Services
Creating insigths by advancing spatial biology
Molecular histology is key to providing spatial understanding of cellular interactions and disease biology in complex tissue samples. The specific cellular localization of RNA molecules, proteins and protein interactions confer important knowledge essential for interpreting biological activity and mechanisms.
Bioneer’s Molecular Histology laboratory is equipped to support advanced immunohistochemistry (IHC) analyses, RNA in situ hybridization (ISH), and in situ Proximity Ligation Assays (isPLA)
To provide insights to our customers, we have implemented a state-of-the-art, versatile analytical workflow using automated processes and AI/ML-guided interpretations of read-outs.
We develop and perform custom-designed fluorescence multiplex assays for studies on diseased tissues, organoids, and 3D cancer models.
Key benefits of our molecular histology services
- High-resolution spatial analysis of RNA and protein expression
- Automated assays for efficient and reproducible results
- Comprehensive biomarker examination using cutting-edge ISH and IHC technologies
- Custom assay development tailored to specific research needs
- Integrated image analysis and quantification for data-driven insights
Our molecular histology services support various applications, including:
Biomarker expression and validation – Identify and validate RNA and proteins in tissues for disease research and drug development.
Diagnostic studies in disease biology – Investigate the spatial distribution of therapeutic targets in tissue samples from human, mouse, rat, etc.
Mechanism-of-action analyses – Understand how proteins interact at the tissue level.
Cancer research and tumor microenvironment analysis – Examine molecular changes within tumors and their microenvironment for diagnostic and therapeutic assessment.
Preclinical evaluation of drug candidates – Assess cellular changes caused by new therapies in solid tumor cancer models (3D BioSPHEERs).
Inflammation and immune response studies – Characterize immune cell infiltration and cytokine expression in diseased tissue and 3D cancer models.
Advanced technologies and capabilities
In Situ Hybridization (ISH) – Automated
- mRNA and long ncRNA (RNAscope)
- microRNA and short RNA (LNA, miRNAscope)
- circRNAs and splice junctions (BaseScope)
Immunohistochemistry (IHC) – Automated
- Chromogenic and fluorescence-based staining
- Multiplex (4-5) immunofluorescence staining
- In situ Proximity Ligation Assay (isPLA) for protein interactions
Combined ISH & IHC Assays
- RNAscope with immunofluorescence
- LNA with immunofluorescence
- Chromogenic combinations
Sample processing
- Tissue embedding for cryopreservation and paraffin
- Handling small specimens like organoids and cancer spheroids
- Slide scanning and quantitative image analysis
At Bioneer, we can also assist you with the development of 3D cancer cell models and iPSC-derived organoids to achieve drug testing models, seamlessly complementing our molecular histology services.
These images showcase the flexibility of molecular histology techniques for RNA and protein detection
Clockwise from top left: microRNA staining with LNA™ probes in brain organoids; microRNA detection using miRNAscope™ in brain tissue; combined microRNA detection with LNA™ probes, RNAscope®, and immunofluorescence in colon cancer; and four-marker immunofluorescence in cancer heterospheroids.
These images showcase the flexibility of molecular histology techniques for RNA and protein detection
Clockwise from top left: microRNA staining with LNA™ probes in brain organoids; microRNA detection using miRNAscope™ in brain tissue; combined microRNA detection with LNA™ probes, RNAscope®, and immunofluorescence in colon cancer; and four-marker immunofluorescence in cancer heterospheroids.
Contact us for a free consultation
For more information or to discuss your project, you can also contact Molecular Histologist Boye Schnack Nielsen directly at bsn@bioneer.dk.
