Optimized fed-batch fermentation: A cost-effective, scalable solution for high-yield recombinant protein production

March, 2025

E. coli expression remains a mainstay for producing recombinant proteins, widely applied in therapeutic development and bio-solutions. 

At Bioneer, we’ve refined this foundational method through a streamlined, highly adaptable fed-batch fermentation process using minimal media—ensuring a smooth transition to cGMP manufacturing.

By supporting multiple protein modalities, delivering high yields and minimizing unwanted byproducts, our optimized platform helps accelerate clients’ CMC development and protein production.

Highlights

• • High biomass and yield – Achieves optical densities (OD600) up to 200-300, resulting in significantly higher recombinant protein production.
  • Optimized glucose feeding– A controlled glucose feed prevents acetate buildup, supporting efficient cell growth.
  • Scalable and reproducible– The process is designed for upscaling to cGMP manufacturing, ensuring consistency in production.
  • Applicable to both soluble and insoluble proteins– Demonstrated efficiency in producing inclusion bodies and soluble proteins.

This process provides a cost-effective, scalable solution for biotech and pharmaceutical companies needing high-yield protein production.