Immune Cell Isolation & Sorting
Early phase immunological research begins with precise immune cell isolation and sorting, and we are equipped to meet your needs.
We specialize in the isolation of PBMCs and various immune subsets, including T cells/Treg cells, monocytes, NK cells, and neutrophils and we ensure the purity and viability of isolated cells.
We utilize well established equipment such as the AutoMACS and BD FACSAria III for cell sorting and isolation. Whether you require sorting of cells from specific tissues or isolation of specialized immune cell populations, we have the expertise to deliver precision and efficiency while ensuring consistent and reliable results. Trust us to tailor our services to your unique research objectives, enabling you to accelerate development of your early drug pipeline.
Immune Cell Differentiation
Understanding immune cell differentiation is paramount for immunological research outcomes and for better understanding the impact of your drug candidates.
With a multidisciplinary approach and advanced techniques, we delve deep into the mechanisms governing immune cell fate determination. Whether you are investigating the role of dendritic cells in shaping immune responses or deciphering the plasticity of macrophage polarization, we offer tailored solutions to meet your research needs and gain insights into immune cell differentiation dynamics.
We specialize in deciphering the differentiation pathways of key immune cell populations, including dendritic cells with their pro- and anti-inflammatory phenotypes, as well as macrophages exhibiting M1/M2 polarization. Our expertise also extends to analyze complexities of regulatory T cells (Treg cells).
ELISA Development
ELISA is a crucial method for detecting and quantifying specific proteins. Bioneer offers a comprehensive range of custom ELISA services for applications such as assay development and validation. Our expertise in antibody and antigen applications helps to reduce development time and enhance your assay results.
Proliferation Assays
Our proliferation assays offer insight into the dynamic growth patterns of immune cells in response to various stimuli. Utilizing state-of-the-art techniques, we accurately measure cell division rates, providing valuable data for drug development and immunotherapy research.
1-Way/2-Way MLR Assays
Our Mixed Lymphocyte Reaction (MLR) assays enable the assessment of immune cell interactions, which is crucial for understanding immune responses in diverse contexts. Whether investigating allogeneic reactions or assessing immunomodulatory effects, our MLR assays provide valuable insights into cellular communication and function
Cytokine Release Assays
Our cytokine release assays measure the secretion of the key immune signalling molecules in response to stimuli. By quantifying cytokine profiles, we evaluate immune activation or inhibition, inflammation, and therapeutic efficacy, guiding the development of immunomodulatory therapies.
Immune Phenotyping
Our immune phenotyping services characterize immune cell populations based on surface markers and functional profiles. By employing flow cytometry, immuno other advanced techniques, we provide comprehensive immune cell profiling, essential for understanding immune dysregulation in diseases.
Flow Cytometry-Based Quantification
Our flow cytometry-based quantification assay can accurately quantify various biomolecules such as receptors and other binding sites on cells. This assay utilizes flow cytometry and antibody labeling technology and can be used to distinguish between absolute number of targets either intracellularly or on the cell surface. This application can be used to determine the ratio between multiple therapeutic targets for combination treatment in drug development.
Killing Assays
Our killing assays assess the cytotoxic capabilities and Antibody Dependent Cellular Cytotoxicity (ADCC) of immune cells, such as cytotoxic T cells, NK cells, and neutrophils. By quantifying target cell death, we elucidate immune cell-mediated cytotoxicity, essential for understanding immune responses against pathogens and tumors.
Phagocytosis Assays
Our phagocytosis assays, either by flow cytometry or image-based analysis, can quantify the engulfment of particles or pathogens by phagocytic cells, such as macrophages, neutrophils or microglia. By measuring phagocytic activity, we assess immune cell function and innate immune responses to infection and inflammation.
Safety and Toxicity Assessment
Our safety and toxicity assessment services evaluate the immunotoxic effects of compounds and therapeutics. By employing a range of assays, we identify potential adverse effects on immune cells, guiding safety evaluations in drug development and regulatory submissions.
Cytokine storm evaluation
Our cytokine storm evaluation services investigate hyperinflammatory responses associated with severe immune reactions. By quantifying cytokine levels and immune cell activation markers, we provide insights into cytokine storm syndromes and potential therapeutic interventions.
ATMP Potency Assays
Our advanced therapy medicinal product (ATMP) potency assays assess the efficacy of advanced cell-based therapies such as iPSC-derived cell types, Mesenchymal Stromal Cells (MSCs) and CAR-T cells. By measuring relevant endpoints, we ensure the potency and consistency of ATMPs, critical for their clinical efficacy and regulatory approval.
For further information
please contact:
Philip Carlsen, Ph.D.
Business Development Manager
