Our toolbox
Molecular Histology
We embed tissues for cryopreservation and in paraffin. Molecular histology analysis includes slide scanning in bright field and fluorescence scanners, and quantitative image analysis. Bioneer offers advanced research platforms including:
- In situ hybridization (ISH) – Automated
- mRNA and long ncRNA (RNAscope)
- microRNA and short RNA (LNA, miRNAScope)
- circRNAs and splice junctions (BaseScope)
- Immunohistochemistry (IHC) – Automated
- Chromogenic
- Fluorescence
- 4-5 multiplexing
- Combined assays IHC and ISH
- Confocal slide scanning
- Image analysis and quantification
- Embedding tissues in OCT compound and in paraffin
* Bioneer is an Exiqon Center of Excellence using LNA™ probes for in situ hybridization and a certified provider of ACD’s state-of-the-art RNAscope®, BaseScope® and miRNAscope™ assays for in situ hybridization.
Molecular Profiling
- Biomarker profiling on cell cultures or cancer spheroids using the Luminex platform to measure e.g. chemokine and cytokine levels or other soluble biomarkers.
- RNA expression profiling using RT-qPCR or RNAseq, i.e. whole transcriptomic analysis, on total RNA extracts from tissue biopsies and cell cultures.
Flow cytometry analysis and cell sorting
- Population
Functional drug screening assays.
- Viability assays using ATP-measurement or reductive capacity of cells as
Viability assay
Apoptosis assay
Cancer-cell specific killing assays
- Reporter cancer cells can be constructed and used to monitor cancer-cell specific killing.
For further information
please contact:
