Today, July 16, 2020, 10 years have passed, since Bioneer’s molecular histology expert Dr. Boye Schnack Nielsen and co-authors published one of the first papers about the locked nucleic acid (LNA) probe technology and the use thereof for in situ hybridization detection of microRNAs.

The LNA technology is very useful for specific detection of miRNA and makes use of nucleic acid analogues called Locked Nucleic Acids, in which the ribose ring is locked into a certain conformation. In addition to in situ hybridization, the technology can also be used for microarray profiling, qPCR, and micro-magnetic beads. The LNAs show higher basepairing affinity than both DNA and RNA, and the LNA oligos can be optimized for higher sensitivity and less unspecific binding.

Read the original 2010 Methods’ paper here (e-publication available July 16, 2010).

At Bioneer, the LNA technology is used for detection of multiple microRNAs via both chromogenic and fluorescence detection. In addition, we use use Roche’s Ventana Discovery Ultra instrument for automation. If you want to know more about the LNA technology, contact Research and Business Manager Boye Schnack Nielsen via email or call +45 45 160 444.

To get an overview of Bioneer’s services within Molecular Histology, follow the link.